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Published on: December 3, 2011
A rapid method for PCR detection of bovine materials in animal feedstuffs
R F Wang1, M J Myers, W Campbell
1Microbiology Division, National Center for Toxicological Research, Jefferson, AR 72079, USA. rwang@nctr.fda.gov
Abstract:
Rapid identification of bovine materials in animal feedstuffs is essential for effective control of a potential source of bovine spongiform encephalopathy. We have developed a rapid method for the detection of the presence of bovine materials in animal feeds. Animal feed samples were prepared by a Chelex-100 treatment method, then subjected to polymerase chain reaction (PCR) detection. The assay can be completed in 2 h including 30 min for sample preparation, 35-65 min for PCR cycling and 30 min for gel electrophoresis. This method is not only rapid, simple and consistent, but also avoids a hazardous waste disposal issue associated with a previously described guanidine thiocyanate (GuSCN) extraction-PCR method.
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