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Related Experiment Videos

Transcription elongation factor SII.

M Wind1, D Reines

  • 1Department of Biochemistry and Graduate Program in Genetics & Molecular Biology, Emory University School of Medicine, Atlanta, GA 30322, USA.

Bioessays : News and Reviews in Molecular, Cellular and Developmental Biology
|March 21, 2000
PubMed
Summary

Transcription factor SII (Standard Inositol-trisphosphate-binding protein) assists RNA polymerase II in overcoming elongation roadblocks. This protein stimulates RNA cleavage, aiding in mRNA synthesis and error correction during transcription.

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Area of Science:

  • Molecular Biology
  • Gene Expression
  • Biochemistry

Background:

  • RNA polymerase II (pol II) mediated transcription involves complex regulation with capping, splicing, and polyadenylation.
  • Elongation factors enhance pol II transcription efficiency.
  • Standard Inositol-trisphosphate-binding protein (SII) is a known elongation factor.

Purpose of the Study:

  • To elucidate the mechanism of action for SII in RNA transcription.
  • To investigate the role of SII in overcoming elongation blocks.
  • To understand the biological significance of SII in mRNA synthesis.

Main Methods:

  • High-resolution structural analysis of SII.
  • Mutagenesis studies to refine the mechanism of action.
  • In vitro biochemical assays.

Related Experiment Videos

  • Molecular genetic experiments in yeast.

    • Main Results:

    • SII facilitates pol II in bypassing elongation blocks by stimulating intrinsic nascent RNA cleavage.
    • This cleavage activity also enables the removal of misincorporated RNA bases.
    • Yeast genetic studies indicate SII's general role in mRNA synthesis.

    Conclusions:

    • SII is a crucial elongation factor that enhances RNA polymerase II processivity.
    • SII's mechanism involves stimulating RNA cleavage for overcoming transcriptional pauses and correcting errors.
    • Further research is needed to understand the functional differences among vertebrate SII isoforms and their regulatory roles in multicellular organisms.