Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Influenza encephalopathy and encephalitis].

E Nobusawa1

  • 1Department of Virology, Nagoya City University, Medical School.

No to Hattatsu = Brain and Development
|March 21, 2000
PubMed
Summary

Neurovirulent influenza A virus (WSN) infects mouse brains, unlike human strains causing encephalopathy. This suggests altered hemagglutinin cleavage and receptor binding may influence influenza neurotropism and disease severity.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Influenza gain-of-function experiments: their role in vaccine virus recommendation and pandemic preparedness.

mBio·2014
Same author

Amino-acid change on the antigenic region B1 of H3 haemagglutinin may be a trigger for the emergence of drift strain of influenza A virus.

Epidemiology and infection·2004
Same author

Analysis of the desialidation process of the haemagglutinin protein of influenza B virus: the host-dependent desialidation step.

The Journal of general virology·2002
Same author

Change in receptor-binding specificity of recent human influenza A viruses (H3N2): a single amino acid change in hemagglutinin altered its recognition of sialyloligosaccharides.

Virology·2000
Same author

Surveillance of influenza viruses isolated from travellers at Nagoya International Airport.

Epidemiology and infection·2000
Same author

Variation in response among individuals to antigenic sites on the HA protein of human influenza virus may be responsible for the emergence of drift strains in the human population.

Virology·2000

Area of Science:

  • Virology
  • Neuroscience
  • Pathology

Context:

  • Influenza A virus pathogenicity and neurovirulence depend on hemagglutinin (HA) cleavage by proteases.
  • A neurovirulent strain (WSN) adapted to mouse brains can replicate in various cell types, including neuroblastoma cells, without exogenous trypsin.
  • Human influenza viruses associated with acute encephalopathy do not exhibit neurovirulence in mice or replicate in human neural cell lines.

Purpose:

  • To investigate the neurovirulence of influenza A virus WSN strain in a mouse model.
  • To compare the in vitro growth characteristics of WSN and human influenza strains in neural cells.
  • To explore the potential link between altered receptor binding specificity and the occurrence of influenza encephalitis/encephalopathy.

Summary:

  • Intracerebral inoculation of WSN in mice resulted in viral antigen detection in the substantia nigra and hippocampus.
  • Human influenza viruses from encephalopathy cases did not cause neurological symptoms in mice or grow in human neuroblastoma/glioblastoma cells.
  • A shift in receptor binding specificity, indicated by reduced chicken erythrocyte agglutination since 1991, is being examined for its role in influenza-associated neurological diseases.

Impact:

  • This research highlights differences in neurovirulence between adapted and recent human influenza A strains.
  • Findings suggest that HA cleavage and receptor binding alterations may be critical factors in determining influenza neurotropism.
  • The study underscores the need for further investigation into the mechanisms underlying influenza encephalitis and encephalopathy.

Related Experiment Videos