Related Experiment Videos
Difference in susceptibilities of different cell lines to bilirubin damage
K C Ngai1, C Y Yeung, C S Leung
1Department of Paediatrics, Queen Mary Hospital, University of Hong Kong, China.
Objective:
To investigate if there are differences in susceptibilities to bilirubin toxicity of different cell lines.
Methodology:
A modified 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) method was adopted to study the cytotoxic effect of bilirubin on several commercially available cell lines including human glioblastoma (ATCC CRL 1690, T98G), human neuroblastoma (ATCC HTB-10, SK-N-MC), human liver (ATCC CCL 13, Chang Liver, HeLa markers) and a mouse fibroblast (ATCC CCL-1, NCTC Colon 929).
Results:
Cytotoxicity was observed when certain bilirubin:albumin molar ratios were exceeded in the medium of a cell line in culture. Different cells exhibited different susceptibilities to the cytotoxic effects of bilirubin; neuroblastoma and glioblastoma were most susceptible, fibroblasts were the least vulnerable.
Conclusions:
Our findings have confirmed the clinical impression that different cells sustain different degrees of cytotoxicities caused by bilirubin.