Related Experiment Video
Updated: Aug 15, 2026

Sheathless Capillary Electrophoresis–Mass Spectrometry for Metabolic Profiling of Biological Samples
Published on: October 1, 2016
A new method of capillary electrophoresis for metabolites of coumarin
M Tegtmeier1, A Mühlau, D Dücker
1Department of Pharmaceutical Technology and Biopharmacy, Schaper & Brümmer, Salzgitter, Germany.
Abstract:
The metabolism of coumarin has been very widely investigated compared with other natural compounds. We know today that genetic variations lead to human groups with a high or low coumarin-metabolism. An effective capillary-electrophoresis method has been developed to study these effects on metabolic patterns. The seven most important metabolites can be analysed in one step. Therefore also small volume samples can be examined for phase-I-reactions (hydroxylation of coumarin) as well as for secondary reactions (e.g. glucuronidation or decomposition of the lactone-structure).
More Related Videos
10:17High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry
Published on: April 23, 2019
07:54Capillary Electrophoresis Mass Spectrometry Approaches for Characterization of the Protein and Metabolite Corona Acquired by Nanomaterials
Published on: October 27, 2020
Related Concept Videos
Coulometry: Overview
Controlled-Potential Coulometry: Electrolytic Methods
The chosen potential ensures...
Controlled-Current Coulometry: Coulometric Titration
The fundamental requirements for coulometric titrations are (1) 100% efficiency in the reagent-generating electrode reaction and (2) a stoichiometric and preferably rapid reaction between the generated...
Electrophoresis: Overview
There...
Capillary Electrophoresis: Instrumentation
Capillary Electrophoresis: Applications
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...