Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Castration increases and androgens decrease nitric oxide synthase activity in the brain: physiologic implications.

R Singh1, S Pervin, J Shryne

  • 1Department of Obstetrics and Gynecology, Laboratory of Neuroendocrinology of the Brain Research Institute, University of California School of Medicine, Los Angeles, CA 90095-1740, USA.

Proceedings of the National Academy of Sciences of the United States of America
|March 22, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Comparison of surgical techniques for the treatment of humeral nonunion using the Ilizarov method.

Scientific reports·2025
Same author

Spinal tumours: recommendations of the Polish Society of Spine Surgery, the Polish Society of Oncology, the Polish Society of Neurosurgeons, the Polish Society of Oncologic Surgery, the Polish Society of Oncologic Radiotherapy, and the Polish Society of Orthopaedics and Traumatology.

European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·2023
Same author

Lysine-specific demethylase KDM3A regulates ovarian cancer stemness and chemoresistance.

Oncogene·2017
Same author

Lysine-specific demethylase KDM3A regulates ovarian cancer stemness and chemoresistance.

Oncogene·2016
Same author

Production of hyperpolarized H_{2} molecules from H[over →] atoms in gas-storage cells.

Physical review letters·2015
Same author

Oxidative stress specifically downregulates survivin to promote breast tumour formation.

British journal of cancer·2013

Testosterone and its metabolite dihydrotestosterone (DHT) reduce nitric oxide synthase (NOS) activity in specific rat brain regions. Androgen treatment reversed castration-induced increases in NOS activity and neuronal NOS expression.

Area of Science:

  • Neuroendocrinology
  • Molecular Neuroscience
  • Neuropharmacology

Background:

  • Nitric oxide synthase (NOS) plays a crucial role in neurotransmission and neurovascular regulation.
  • Investigating sex differences and hormonal influences on NOS activity is vital for understanding brain function.

Purpose of the Study:

  • To examine sex differences in brain NOS activity.
  • To determine the effects of androgens (testosterone and DHT) on NOS activity in orchidectomized rats.

Main Methods:

  • Measurement of NOS activity in different rat brain regions.
  • Analysis of neuronal NOS (nNOS) and androgen receptor (AR) mRNA levels via RT-PCR.
  • Immunohistochemical detection of nNOS and AR positive cells.

Main Results:

Related Experiment Videos

  • No significant sex differences in NOS activity were found in gonadectomized rats.
  • Orchidectomy increased NOS activity and nNOS mRNA levels in the hypothalamus, amygdala, and cerebellum.
  • Androgen treatment (testosterone or DHT) reversed these castration-induced increases, suggesting down-regulation of NOS by androgens.

Conclusions:

  • Testosterone, likely via its metabolite DHT, down-regulates NOS activity, nNOS mRNA expression, and the number of nNOS-expressing neurons in the rat hypothalamus.
  • Androgen receptor expression shows a different pattern, suggesting complex regulatory mechanisms.