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Low probe concentration can cause problems in multilocus DNA fingerprinting (cautionary notes III)
T Gerken1, T Lubjuhn, J T Epplen
1Institute for Evolutionary Biology and Ecology, University of Bonn, Germany. tgerken@evolution.uni-bonn.de
Electrophoresis
|March 22, 2000
Abstract:
The power of multilocus DNA fingerprinting depends on the reliability with which the uniqueness of an individual's profile can be demonstrated. This cautionary note stresses the importance of the probe concentration in this procedure. In case of a probe shortage, DNA fragments rich in tandem repeats have the potential to impede hybridization in other parts of the gel, and thus interfere with bands that are part of a DNA fingerprinting profile.