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Updated: Jul 18, 2026

Detection of Nitric Oxide and Superoxide Radical Anion by Electron Paramagnetic Resonance Spectroscopy from Cells using Spin Traps
Published on: August 18, 2012
Quantitative electron paramagnetic resonance and spectrophotometric determination of the free radical
1Institute of Catalysis, Bulgarian Academy of Sciences, Sofia. ndyepr@bas.bg
Abstract:
The first quantitative estimation of the stable free radical 4-hydroxy-2,2,6,6-tetramethylpiperidinyloxy (TEMPOL) simultaneously performed by electron paramagnetic resonance (EPR) and visible spectrophotometric titration is reported. The obtained molar absorptivity of TEMPOL 13.2 +/- 0.1 l(-1) m(-1) cm(-1) at 22,900 cm(-1) may be used as the future criterium about the purity of the EPR active substance. The studied compound may be considered as the second (after 1,1-diphenyl-2-pikrilhydrazil) material calibrated as the primary standard for quantitative EPR spectrometry.
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