Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Stability of corticosteroid patch test preparations.

M Isaksson1, B Gruvberger, L Persson

  • 1Department of Occupational and Environmental Dermatology, Malmö University Hospital, Sweden.

Contact Dermatitis
|March 22, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Monte Carlo method for calculation of the characteristic limits decision threshold and detection limit in low-level radioactivity measurements.

Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine·2021
Same author

Calculation of decision threshold and detection limit in radiometric measurements using a Monte Carlo Method.

Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine·2019
Same author

Prevalence and Pathogenicity of Foot and Root Rot Pathogens of Pea in Southern Scandinavia.

Plant disease·2019
Same author

Infection of Potatoes by Oospores of Phytophthora infestans in Soil.

Plant disease·2019
Same author

Assessment of Soil Suppressiveness to Aphanomyces Root Rot of Pea.

Plant disease·2019
Same author

Root Rot of Pea and Faba Bean in Southern Sweden Caused by Phytophthora pisi sp. nov.

Plant disease·2019
Same journal

Contact Allergy and Allergic Contact Dermatitis From Propylene Glycol and Related Glycols: Cosmetic Skin Sensitisers After All?

Contact dermatitis·2026
Same journal

Acrylate Copolymers/Crosspolymers in Sunscreens: Minimally Allergenic, Without Evidence of Need for Avoidance by (Meth)Acrylate-Sensitized Individuals.

Contact dermatitis·2026
Same journal

AI-Assisted Automated Two-Stage Patch Test Interpretation System Using Vision Transformer.

Contact dermatitis·2026
Same journal

Use of Tralokinumab in a Hairdresser With Severe Chronic Hand Eczema and Occupational Allergic Contact Dermatitis.

Contact dermatitis·2026
Same journal

Occupational Allergic Contact Dermatitis Caused by Cyclohexyl Diethanolamine in a Metalworking Fluid.

Contact dermatitis·2026
Same journal

Fragrance and Preservative Contact Allergens in Cosmetic and Household Cleaning Products in Turkey: Variation by Target Population, Product Type and Manufacturing Origin.

Contact dermatitis·2026
See all related articles

Tixocortol pivalate and budesonide in petrolatum and ethanol were stable for one year across storage conditions. Hydrocortisone-17-butyrate stability varied, with room temperature ethanol solutions degrading within three months.

Area of Science:

  • Dermatology
  • Pharmaceutical Sciences
  • Analytical Chemistry

Background:

  • Topical corticosteroids are crucial in treating inflammatory skin conditions.
  • Understanding the stability of these compounds in various formulations and storage conditions is essential for efficacy and safety.
  • Previous studies, including those by the European Environmental Contact Dermatitis Research Group (EECDRG), have established testing protocols for corticosteroid efficacy.

Purpose of the Study:

  • To assess the stability of tixocortol pivalate, budesonide, and hydrocortisone-17-butyrate (Hc-17-B) in petrolatum and ethanolic solutions.
  • To evaluate the impact of different storage conditions (room temperature, refrigeration, deep freezing) on corticosteroid stability over one year.
  • To compare the stability of individual corticosteroids versus their mixtures.

Related Experiment Videos

Main Methods:

  • Corticosteroids were formulated in petrolatum and ethanolic solutions at concentrations relevant to EECDRG patch testing.
  • Preparations were stored at room temperature, refrigerated, and deep frozen for up to one year.
  • High-performance liquid chromatography (HPLC) was used to quantify corticosteroid concentrations at regular intervals.
  • Stability was defined as a decrease of less than or equal to 20% from the initial concentration.

Main Results:

  • Petrolatum preparations of all three corticosteroids remained stable for the entire one-year study period, regardless of storage conditions.
  • Ethanolic solutions of budesonide and tixocortol pivalate were stable for one year under all tested storage conditions.
  • Hydrocortisone-17-butyrate (1.0% in ethanol) showed stability when deep frozen but degraded within three months at room temperature.

Conclusions:

  • Budesonide and tixocortol pivalate exhibit excellent stability in both petrolatum and ethanol formulations under various storage conditions.
  • Hydrocortisone-17-butyrate stability is formulation and storage-dependent, with ethanol solutions requiring specific conditions (e.g., deep freezing) to maintain integrity long-term.
  • These findings have implications for the formulation, storage, and clinical use of topical corticosteroids to ensure therapeutic effectiveness.