Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Is a functional sarcoplasmic reticulum necessary for preconditioning?

A C Cave1, P B Garlick

  • 1Division of Radiological Sciences, 5th floor, Thomas Guy House, Guy's Hospital, London, UK. alison.cave@kcl.ac.uk

Journal of Molecular and Cellular Cardiology
|March 25, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The low oxygen-carrying capacity of Krebs buffer causes a doubling in ventricular wall thickness in the isolated heart.

Canadian journal of physiology and pharmacology·2005
Same author

Oxidative stress and heart failure.

Archives des maladies du coeur et des vaisseaux·2003
Same author

Divergent biological actions of coronary endothelial nitric oxide during progression of cardiac hypertrophy.

Hypertension (Dallas, Tex. : 1979)·2001
Same author

ATP synthesis during low-flow ischemia: influence of increased glycolytic substrate.

Circulation·2000
Same author

A new system for the metabolic investigation of the isolated, perfused rat heart.

Journal of molecular and cellular cardiology·2000
Same author

Improved functional recovery by ischaemic preconditioning is not mediated by adenosine in the globally ischaemic isolated rat heart.

Cardiovascular research·2000

Ischemic preconditioning (PC) protects the heart, but its link to sarcoplasmic reticulum (SR) calcium release is unclear. This study shows PC remains protective even when SR calcium handling is impaired, suggesting SR calcium release is not essential for PC's protective effects.

Area of Science:

  • Cardiology
  • Physiology
  • Biochemistry

Background:

  • Ischemic preconditioning (PC) offers cardiac protection.
  • Reduced calcium release from the sarcoplasmic reticulum (SR) is linked to PC.
  • The necessity of SR calcium handling for PC remains unproven.

Purpose of the Study:

  • To determine if a functional SR is essential for the protective mechanisms of PC.
  • To investigate the role of SR calcium handling in PC-induced cardioprotection.

Main Methods:

  • Isolated rat hearts were subjected to normoxic perfusion followed by ischemia/reperfusion protocols.
  • SR calcium handling was manipulated using ryanodine (RY) and cyclopiazonic acid (CPA).
  • 31P-NMR spectroscopy assessed energy metabolites (ATP, PCr, Pi) and intracellular pH.

Related Experiment Videos

Main Results:

  • RY and CPA significantly reduced developed pressure, confirming impaired SR calcium handling.
  • PC significantly improved left ventricular developed pressure (LVDP) recovery compared to controls.
  • PC maintained its protective effect on LVDP recovery even with RY and CPA treatment, indicating SR dysfunction did not abolish protection.

Conclusions:

  • Ischemic preconditioning (PC) provides cardiac protection even when sarcoplasmic reticulum (SR) calcium accumulation is inhibited.
  • Changes in SR calcium release are not essential for the cardioprotective effects of PC.
  • These findings challenge the established role of SR calcium handling in the mechanism of PC.