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Published on: July 6, 2014
Serum müllerian-inhibiting substance levels during normal menstrual cycles
1University of Louisville, Louisville, Kentucky, USA. clcook01@gwise.louisville.edu
Objective:
To measure serum levels of müllerian-inhibiting substance (MIS) during the normal menstrual cycle.
Design:
Serum was collected from women during ovulation and the mid-luteal and early follicular phases of the menstrual cycles. It was stored frozen at -80 degrees C until assayed.
Setting:
University of Louisville Departments of Obstetrics and Gynecology and Surgery.
Patient(S):
Twenty healthy women 22-35 years of age with normal menstrual cycles.
Intervention(S):
Blood samples were collected on menstrual cycle day two or three and on the day of LH surge plus one and plus seven or eight.
Main Outcome Measure(S):
Serum MIS levels were measured by using an enzyme-linked immunosorbent assay.
Result(S):
Serum MIS levels ranged from a low of 1.4 +/- 0.9 ng/mL (mean [+/-SD]) in the early follicular phase, peaked mid-cycle at 1.7 +/- 1.1 ng/mL, and decreased to 1.4 +/- 0.9 ng/mL in the mid-luteal phase of the normal menstrual cycle.
Conclusion(S):
Fluctuations in serum MIS levels during the menstrual cycle suggest that MIS may have a regulatory role in folliculogenesis.
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