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Hamster estrogen receptor cDNA: cloning and mRNA expression
1Division of Laboratory Research and Development, Department of Medicine, Charles R. Drew University of Medicine and Science, 1731 East 120th Street, Los Angeles, CA 90059, USA.
The Journal of Steroid Biochemistry and Molecular Biology
|March 25, 2000
Summary
Researchers cloned the full hamster estrogen receptor cDNA, revealing high homology with other species. This finding advances understanding of hormonal carcinogenesis in the hamster kidney tumor model.
Area of Science:
- Endocrinology
- Molecular Biology
- Carcinogenesis Research
Background:
- The estrogen-induced hamster kidney tumor model is crucial for studying hormonal carcinogenesis.
- Previous studies indicated increased estrogen receptor (ER) expression in this model.
- Sequence information for the hamster ER gene was previously unknown.
Purpose of the Study:
- To clone and sequence the full-length hamster estrogen receptor (ER) cDNA.
- To analyze the homology of the hamster ER with other species' ERs.
- To further elucidate mechanisms of hormonal carcinogenesis.
Main Methods:
- Construction of a hamster uterus cDNA library.
- Polymerase chain reaction (PCR) amplification using homology-based primers.
- Cloning, Southern blot analysis, and DNA sequencing of PCR products.
Main Results:
- A full-length 1978 bp hamster ER cDNA was successfully cloned.
- The hamster ER cDNA exhibited high homology (87-91%) with human, rat, and mouse ER cDNA.
- The deduced amino acid sequence showed significant homology (88-93%) with other species' ERs, with 100% homology in the DNA-binding domain (Domain C).
Conclusions:
- The cloned hamster ER provides a valuable tool for studying ER function in hormonal carcinogenesis.
- High sequence homology suggests conserved functions of the estrogen receptor across species.
- This research facilitates deeper investigation into the molecular mechanisms underlying estrogen-driven kidney tumors.
