Midkine enhances early stages of collagen gel contraction

¹Department of Biochemistry and Department of Oral and Maxillofacial Surgery, Nagoya University School of Medicine, Tsurumai-cho, Showa-ku, Nagoya 466-8550, Japan.

|March 25, 2000

Abstract:

Midkine (MK) is a heparin-binding growth/differentiation factor implicated in the control of development and repair of various tissues. To investigate the roles of MK in embryogenesis and in regulation of wound healing, we utilized a system of collagen gel contraction by dermal fibroblasts, which provides an in vitro model for studying fibroblast-collagen interactions important in various physiological and pathological phenomena. MK enhanced gel contraction 8-24 h after plating, and its effect was inhibited by anti-MK antibody. The effect was reduced after 48 h, while TGF-beta continued to be active in the later stage. Morphologically, MK-treated fibroblasts tended to be elongated more frequently than control fibroblasts. The effect of MK in the early stage of gel contraction suggests a role of MK as a modulator of cell-matrix interactions.

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Abstract:

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