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Updated: Jun 20, 2026

On-chip Isotachophoresis for Separation of Ions and Purification of Nucleic Acids

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Published on: March 2, 2012

Preparative-scale isoelectric focusing separation of enantiomers using a multicompartment electrolyzer with

P Glukhovskiy¹, T A Landers, G Vigh

¹Chemistry Department, Texas A&M University, College Station 77842-3012, USA.

Electrophoresis

|March 25, 2000

Summary

This summary is machine-generated.

Researchers achieved high-purity separation of dansyl phenylalanine enantiomers using a novel multicompartment electrolyzer and cyclodextrin. This method offers a scalable approach for chiral separations, despite challenges with salt content.

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Separation of Bioactive Small Molecules, Peptides from Natural Sources and Proteins from Microbes by Preparative Isoelectric Focusing (IEF) Method

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Published on: June 14, 2020

Area of Science:

Analytical Chemistry
Separation Science
Chiral Chemistry

Background:

Preparative-scale enantiomer separation is crucial for pharmaceuticals and fine chemicals.
Isoelectric focusing (IEF) is a powerful separation technique, but its preparative application for chiral compounds requires optimization.
Cyclodextrins are widely used as chiral selectors in separation science.

Purpose of the Study:

To evaluate the IsoPrime multicompartment electrolyzer for preparative-scale chiral separation.
To determine the efficacy of hydroxypropyl beta-cyclodextrin as a resolving agent in this system.
To optimize separation conditions for dansyl phenylalanine enantiomers.

Main Methods:

Utilized a multicompartment electrolyzer with isoelectric membranes.
Employed hydroxypropyl beta-cyclodextrin as the chiral resolving agent.
Systematically adjusted pH gradients between membranes for separation optimization.

Main Results:

Achieved >99.9% enantiomeric excess for dansyl phenylalanine enantiomers.
Demonstrated preparative-scale separation at approximately 0.1 mg/h.
Identified high salt content in the resolving agent as a limiting factor for field strength.

Conclusions:

The IsoPrime electrolyzer is effective for preparative chiral separations.
Optimization of pH gradients facilitated rapid method development.
Future work should address challenges related to sample conductivity for enhanced efficiency.