Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A simple method for human peripheral blood monocyte isolation.

M C de Almeida1, A C Silva, A Barral

  • 1Departamento de Patologia, FCS, Universidade de Brasília, DF, Brasil.

Memorias Do Instituto Oswaldo Cruz
|March 25, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Biomarkers in the pathogenesis and diagnosis of Crohn's perianal fistulas: a systematic review.

Techniques in coloproctology·2026
Same author

Initial experience in colorectal robotic surgery in a university hospital: the first 100 consecutive cases using Hugo™ RAS platform.

Techniques in coloproctology·2026
Same author

Diagnosis and risk factors associated with Schistosoma mansoni infection in a population of an endemic area of Northeastern Brazil.

Brazilian journal of biology = Revista brasleira de biologia·2025
Same author

Optimising patient positioning in magnetic resonance imaging for image Co-registration in radiosurgery planning.

Radiography (London, England : 1995)·2025
Same author

Maximising olfactory deposition of a valproic acid (VPA)-loaded nanostructured lipid carriers (NLC) formulation.

International journal of pharmaceutics·2025
Same author

Clinical, laboratory and epidemiological aspects of Cryptosporidium parvum and Cryptosporidium hominis infection: a integrative review.

Brazilian journal of biology = Revista brasleira de biologia·2025
Same journal

Chromosome level genome assembly of the World Health standards Leishmania (Viannia) guyanensis M4147 and L. (V.) shawi M8408 using a hybrid sequencing approachFIRST REVIEW ROUND - REVIEWERS' COMMENTSREVIEWERS' COMMENTS.

Memorias do Instituto Oswaldo Cruz·2026
Same journal

Uptake and survival of Leishmania amazonensis in Acanthamoeba: an adequate model organism?FIRST REVIEW ROUND - REVIEWERS' COMMENTSREVIEWERS' COMMENTSREVIEWERS' COMMENTS.

Memorias do Instituto Oswaldo Cruz·2026
Same journal

Dengue virus serotype 4 infection in human fatal cases: histopathological investigationFIRST REVIEW ROUND - REVIEWERS COMMENTSREVIEWERS COMMENTS.

Memorias do Instituto Oswaldo Cruz·2026
Same journal

Novel epitope-based diagnostic probes selected by phage display for the serological detection of HDV

Memorias do Instituto Oswaldo Cruz·2026
Same journal

Coexistence of monogamy and polygyny in Triatoma infestans: fine-scale genealogical structure reveals complex social structures within domestic colonies in the Bolivian Chaco

Memorias do Instituto Oswaldo Cruz·2026
Same journal

Is Wolbachia a sustainable component for dengue control?FIRST REVIEW ROUND - REVIEWERS COMMENTSREVIEWERS COMMENTS.

Memorias do Instituto Oswaldo Cruz·2026
See all related articles

A simple Percoll gradient method efficiently isolates highly functional human monocytes from blood. This cost-effective technique uses basic lab equipment for convenient monocyte isolation.

Area of Science:

  • Immunology
  • Cell Biology
  • Hematology

Background:

  • Monocytes are crucial immune cells involved in host defense.
  • Efficient isolation of functional monocytes is essential for immunological research and therapeutic applications.

Purpose of the Study:

  • To present a straightforward and cost-effective method for isolating highly enriched human monocytes.

Main Methods:

  • Utilized a Percoll gradient centrifugation technique.
  • Applied the method to whole blood or buffy coat cells.

Main Results:

  • Achieved high numbers of fully functional human monocytes.
  • The isolation process is simple and requires basic laboratory equipment.

Related Experiment Videos

Conclusions:

  • The described Percoll gradient method offers a convenient and economical approach for obtaining pure, functional human monocytes.
  • This technique is suitable for both research and potential clinical applications requiring monocyte isolation.