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An in vitro system from Plasmodium falciparum active in endogenous mRNA translation
A Ferreras1, L Triana, H Correia
1Centro de Investigaciones Biomédicas, Facultad de Ciencias de la Salud, Universidad de Carabobo, Maracay, Aragua, Venezuela.
Memorias Do Instituto Oswaldo Cruz
|March 25, 2000
Abstract:
An in vitro translation system has been prepared from Plasmodium falciparum by saponin lysis of infected-erythrocytes to free parasites which were homogeneized with glass beads, centrifuged to obtain a S-30 fraction followed by Sephadex G-25 gel filtration. This treatment produced a system with very low contamination of host proteins (<1%). The system, optimized for Mg2+ and K+, translates endogenous mRNA and is active for 80 min which suggests that their protein factors and mRNA are quite stable.