Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Tissue-engineered valved conduits in the pulmonary circulation.

U A Stock1, M Nagashima, P N Khalil

  • 1Department of Cardiovascular Surgery, Children's Hospital, Boston, MA 02115, USA.

The Journal of Thoracic and Cardiovascular Surgery
|March 25, 2000
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Are we overtreating patients with malignant colorectal polyps? A 5-year review of the ACPGBI position statement.

Annals of the Royal College of Surgeons of England·2021
Same author

One size does not fit all: reaching agricultural workers in South Africa with TB services.

The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease·2021
Same author

Quality of life in breast cancer survivors: An assessment of international breast cancer dragon boat racers.

Lymphology·2021
Same author

Telehealth sounds a bit challenging, but it has potential: participant and physiotherapist experiences of gym-based exercise intervention for Achilles tendinopathy monitored via telehealth.

BMC musculoskeletal disorders·2021
Same author

Creating a model to predict time intervals from induction of labor to induction of anesthesia and delivery to coordinate workload.

International journal of obstetric anesthesia·2021
Same author

Vaccine implementation factors affecting maternal tetanus immunization in low- and middle-income countries: Results of the Maternal Immunization and Antenatal Care Situational Analysis (MIACSA) project.

Vaccine·2020

Tissue-engineered pulmonary valved conduits made from sheep cells and biodegradable polymers show promising function. These innovative grafts demonstrate cellular growth and remodeling, offering a potential alternative to traditional valves.

Area of Science:

  • Regenerative Medicine
  • Biomaterials Science
  • Cardiovascular Engineering

Background:

  • Current bioprosthetic and mechanical valves have limitations in growth, repair, and remodeling.
  • Tissue engineering offers a potential solution to create dynamic, living vascular grafts.

Purpose of the Study:

  • To evaluate the feasibility of creating 3-leaflet, valved, pulmonary conduits using autologous ovine vascular cells and biodegradable polymers.
  • To assess the in vivo function and tissue remodeling of these engineered constructs.

Main Methods:

  • Harvested ovine carotid artery endothelial and medial cells.
  • Constructed composite scaffolds of polyglycolic acid and polyhydroxyoctanoates into conduits with leaflets.
  • Seeded constructs with autologous cells and implanted them to replace the pulmonary valve and main pulmonary artery in sheep.

Related Experiment Videos

Main Results:

  • Seeded constructs showed no thrombus formation and mild, nonprogressive regurgitation up to 24 weeks.
  • Histology revealed organized, viable tissue with increasing cellular and extracellular matrix content.
  • The unseeded control construct developed significant thrombus formation by 4 weeks.

Conclusions:

  • Valved conduits engineered from autologous cells and biodegradable matrices can successfully function in the pulmonary circulation.
  • The observed progressive tissue formation indicates ongoing remodeling for at least 6 months post-implantation.