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Advanced method for oligonucleotide deprotection
S A Surzhikov1, E N Timofeev, B K Chernov
1Joint Human Genome Program, Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 117984 Moscow, Russia.
Nucleic Acids Research
|March 29, 2000
Abstract:
A new procedure for rapid deprotection of synthetic oligodeoxynucleotides has been developed. While all known deprotection methods require purification to remove the residual protective groups (e.g. benzamide) and insoluble silicates, the new procedure based on the use of an ammonia-free reagent mixture allows one to avoid the additional purification steps. The method can be applied to deprotect the oligodeoxynucleotides synthesized by using the standard protected nucleoside phosphoramidites dG(iBu), dC(Bz)and dA(Bz).