Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Endometrial suppressor cells in beef cattle.

S A Shipp1, E C Segerson

  • 1Department of Animal Science, North Carolina A&T State University, Greensboro, USA.

Theriogenology
|March 29, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Selenium status and phytohemagglutinin-stimulated T lymphocytes from beef calves.

Biological trace element research·2013
Same author

High-density ovine endometrial cells exhibit natural killer activity during early pregnancy.

Theriogenology·2001
Same author

Immunosuppressive macromolecules of endometrial and conceptus origins in livestock species.

Journal of reproductive immunology·2000
Same author

A porcine intrauterine 4-mda component with transforming growth factor-beta activity suppresses natural killer cell responses.

Journal of animal science·2000
Same author

Suppressor activity of bone marrow cells and localization of fluorescent-labeled bone marrow cells within ovine endometrial tissue.

Journal of animal science·2000
Same author

Ovine endometrial cells fail to lyse K-562 target cells: a preliminary investigation.

Theriogenology·2000

Bovine endometrial cells from cyclic and pregnant cows suppress lymphocyte responses and release a transforming growth factor-beta (TGF-beta) associated factor. These suppressor cells are found in low and high-density fractions.

Area of Science:

  • Reproductive immunology
  • Cell biology

Background:

  • The maternal immune system must tolerate the semi-allogeneic fetus during pregnancy.
  • Endometrial cells play a crucial role in modulating the uterine immune environment.

Purpose of the Study:

  • To investigate the immunomodulatory properties of bovine endometrial cells.
  • To identify and characterize suppressor cells within the bovine endometrium.
  • To determine the nature of the immunosuppressive factor released by these cells.

Main Methods:

  • Post-mortem recovery of endometrial cells from cyclic and pregnant cattle.
  • In vitro lymphocyte proliferation assays to assess suppression.
  • Analysis of suppressor factor for transforming growth factor-beta (TGF-beta) activity.
  • Percoll density gradient centrifugation to fractionate endometrial cells.

Related Experiment Videos

Main Results:

  • Endometrial cells from both cyclic and pregnant cows exhibited immunosuppressive activity.
  • A suppressor factor released into the culture medium was identified and showed TGF-beta activity.
  • Fractionation revealed that both low- (1.01 g/mL) and high-density (1.095 g/mL) cells possessed suppressor activity.

Conclusions:

  • The bovine endometrium harbors both low- and high-density suppressor cells.
  • These cells release an immunosuppressive factor associated with TGF-beta.
  • This finding contributes to understanding uterine immune regulation in cattle pregnancy.