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Related Experiment Videos

Low dose insemination in synchronized gilts.

C Krueger1, D Rath, L A Johnson

  • 1Institute of Animal Science and Animal Behaviour, Mariensee, Neustadt, Germany.

Theriogenology
|March 29, 2000
PubMed
Summary

Reducing sperm concentration for pig insemination to 10 million sperm per gilt using surgical deep intra-uterine insemination (IUI) maintained fertilization and litter size. This low-volume method proved effective without compromising reproductive outcomes.

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Area of Science:

  • Reproductive Biology
  • Animal Science
  • Veterinary Medicine

Background:

  • Conventional pig insemination requires high sperm doses (2-3 x 10^9 sperm/dose).
  • High-speed sperm sorting yields limited numbers (5-6 million sperm/hour).
  • Surgical deep intra-uterine insemination (IUI) offers potential for reduced sperm usage.

Purpose of the Study:

  • To determine the minimal effective sperm concentration for pig IUI.
  • To assess the impact of low insemination volumes on fertilization and litter size.
  • To evaluate embryo development post-insemination with reduced sperm counts.

Main Methods:

  • Semen from 3 boars was diluted to various concentrations (1x10^6 to 5x10^8 sperm/0.5 ml).
  • Prepuberal gilts underwent surgical IUI at specific times post-hCG treatment.

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  • Embryo recovery, morphological evaluation, and in vitro culture were performed in a second trial.

    • Main Results:

    • Pregnancy and farrowing rates were unaffected down to 10 million sperm/gilt.
    • No significant differences in litter size or piglet viability were observed.
    • Fertilization rates were comparable across groups, except for the lowest concentration (1x10^6 sperm/horn).
    • Embryo development to morulae and blastocysts was similar to controls.

    Conclusions:

    • A minimum of 10 million sperm per gilt is sufficient for successful surgical deep IUI.
    • Low insemination volumes are effective in pigs, maintaining reproductive efficiency.
    • This approach reduces sperm requirements without negatively impacting embryo development or litter outcomes.