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Related Experiment Videos

Evidence for multiple prolactin receptor transcripts in the turkey.

G R Pitts1, S You, D N Foster

  • 1University of Virginia, Department of Medicine, Charlottesville 22908, USA. grp8n@virginia.edu

Poultry Science
|March 29, 2000
PubMed
Summary

This study identified multiple prolactin receptor (PRL-R) mRNA transcript isoforms in turkeys, with varying sizes and tissue distribution. PRL-R mRNA levels were highest in laying hen pineal glands and incubating hen oviducts, suggesting a link to reproductive status.

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Area of Science:

  • Molecular Biology
  • Endocrinology
  • Avian Biology

Background:

  • Multiple prolactin receptor (PRL-R) mRNA transcript isoforms are known in mammals.
  • Conflicting reports exist regarding the number of avian PRL-R isoforms.
  • The relationship between PRL-R mRNA abundance and reproductive status in birds is not well understood.

Purpose of the Study:

  • To investigate the existence of multiple turkey PRL-R transcript isoforms.
  • To determine the tissue distribution of these isoforms.
  • To explore the correlation between PRL-R mRNA abundance and reproductive status in turkeys.

Main Methods:

  • Reverse transcriptase polymerase chain reaction (RT-PCR) to generate turkey PRL-R cDNA fragments.
  • Northern blot analysis using turkey and chicken PRL-R probes to detect transcript sizes and tissue expression.

Related Experiment Videos

  • Examination of PRL-R transcript isoform expression across different reproductive states in turkeys.

    • Main Results:

    • Two turkey PRL-R cDNA fragments showed high similarity to mammalian and avian PRL-R.
    • Northern blot analysis revealed multiple PRL-R mRNA transcripts (3.1-kb, 1.5-kb, 10.7-kb, and 7.3-kb) in various turkey tissues including liver, oviduct, testes, pineal gland, infundibulum, magnum, isthmus, kidney, and intestine.
    • PRL-R mRNA levels were significantly higher in laying hen pineal glands and incubating hen oviducts.

    Conclusions:

    • This study provides the first evidence for multiple prolactin receptor mRNA transcript isoforms in turkeys.
    • The identified isoforms exhibit distinct tissue-specific expression patterns.
    • PRL-R mRNA abundance appears to be influenced by the reproductive status of the turkey, particularly in the pineal gland and oviduct.