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Related Experiment Videos

Replication of staphylococcal multiresistance plasmids.

N Firth1, S Apisiridej, T Berg

  • 1School of Biological Sciences, University of Sydney, Sydney, New South Wales 2006, Australia.

Journal of Bacteriology
|March 29, 2000
PubMed
Summary

Large staphylococcal multiresistance plasmids utilize a shared, evolutionarily related theta-mode replication system. This finding unifies previously distinct plasmid groups, revealing common replication strategies in Staphylococcus.

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Area of Science:

  • Molecular Biology
  • Microbiology
  • Genetics

Background:

  • Staphylococcal multiresistance plasmids are categorized into three main groups based on structure and function.
  • Understanding the replication mechanisms of these plasmids is crucial for comprehending their persistence and spread.

Purpose of the Study:

  • To analyze and compare the replication functions of representative plasmids from the three major staphylococcal multiresistance plasmid groups.
  • To investigate the evolutionary relationships and functional roles of replication initiation genes and associated upstream genes.

Main Methods:

  • Comparative nucleotide sequence analysis of replication initiation genes (rep) and upstream open reading frames (orfs).
  • Construction and functional analysis of minireplicons to assess the role of specific genes in plasmid stability.

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  • Sequence similarity searches against databases of plasmids from various gram-positive genera.

    • Main Results:

    • Replication initiation genes from pSK1, pSK41, and pI9789::Tn552 are evolutionarily related to each other and to other gram-positive plasmids.
    • The pSK1 plasmid's orf245 is essential for its segregational stability, a feature absent in the conjugative pSK41 plasmid.
    • Many large multiresistance plasmids in staphylococci share a common, evolutionarily related theta-mode replication system.

    Conclusions:

    • Despite previous assumptions of unrelatedness, major staphylococcal multiresistance plasmids employ a conserved theta-mode replication strategy.
    • The orf245 gene plays a significant role in the stability of certain staphylococcal replicons.
    • This conserved replication system likely contributes to the successful dissemination of antibiotic resistance genes in staphylococcal populations.