Jove
Visualize
Contact Us

Related Experiment Videos

Protective DNA immunization against Chlamydia pneumoniae.

C Svanholm1, L Bandholtz, E Castaños-Velez

  • 1Microbiology and Tumorbiology Center, Department of Pathology, Karolinska Institute, Stockholm, Sweden.

Scandinavian Journal of Immunology
|March 29, 2000
PubMed
Summary

DNA vaccination with heat shock protein 60 (HSP-60) protects mice against Chlamydia pneumoniae infection by inducing cell-mediated immunity, not antibodies. This approach offers a promising strategy for developing new vaccines against bacterial infections.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Differential expression of genes encoding tight junction proteins in colorectal cancer: frequent dysregulation of claudin-1, -8 and -12.

International journal of colorectal disease·2006
Same author

Expression of the cathelicidin LL-37 is modulated by short chain fatty acids in colonocytes: relevance of signalling pathways.

Gut·2003
Same author

Hsp90 binds CpG oligonucleotides directly: implications for hsp90 as a missing link in CpG signaling and recognition.

Cellular and molecular life sciences : CMLS·2003
Same author

Adjuvant modulation of the immune responses and the outcome of infection with Chlamydia pneumoniae.

Clinical and experimental immunology·2002
Same author

Lung restricted T cell receptor AV2S3+ CD4+ T cell expansions in sarcoidosis patients with a shared HLA-DRbeta chain conformation.

Thorax·2002
Same author

IFN-alpha beta-dependent, IFN-gamma secretion by bone marrow-derived macrophages controls an intracellular bacterial infection.

Journal of immunology (Baltimore, Md. : 1950)·2001
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Area of Science:

  • Immunology
  • Microbiology
  • Vaccinology

Background:

  • Chlamydia pneumoniae is a significant human pathogen causing respiratory infections.
  • Effective vaccines against C. pneumoniae are lacking.
  • Heat shock protein 60 (HSP-60) is a potential target for vaccine development.

Purpose of the Study:

  • To evaluate the efficacy of DNA vaccination using the HSP-60 gene of C. pneumoniae for protection against bacterial infection in mice.
  • To investigate the immune mechanisms underlying DNA vaccine-induced protection.

Main Methods:

  • Intranasal (i.n.) and intradermal (i.d.) DNA vaccination of C57Bl/6 mice with plasmids encoding HSP-60 (pHSP-60).
  • Assessment of bacterial load in lungs, disease severity, antibody responses (IgG), and cytokine production (IFN-gamma).

Related Experiment Videos

  • Evaluation of vaccine efficacy in knockout mice lacking specific immune components (IFN-gamma receptor, MHC class II, CD4+, CD8+ T cells).

    • Main Results:

    • Intranasal pHSP-60 vaccination significantly reduced C. pneumoniae load and disease severity in mice.
    • Protective immunity was associated with increased IFN-gamma production and T-cell responses, not specific antibodies.
    • Protection was dependent on both CD4+ and CD8+ T cells, with CD4+ T cells alone potentially worsening the outcome.
    • Intranasal administration of IFN-gamma gene alone also conferred protection.

    Conclusions:

    • DNA vaccination targeting HSP-60 via intranasal delivery is effective in protecting mice against C. pneumoniae infection.
    • The protective mechanism relies on cell-mediated immunity, particularly involving interferon-gamma and T cells.
    • This study highlights the potential of DNA vaccines and IFN-gamma in combating C. pneumoniae infections.