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Related Experiment Videos

PapD-like chaperones and pilus biogenesis.

F G Sauer1, S D Knight, G J Waksman and

  • 1Departments of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO 63110, USA.

Seminars in Cell & Developmental Biology
|March 29, 2000
PubMed
Summary
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Gram-negative bacteria use PapD-like chaperones to assemble adhesive pili. These chaperones stabilize subunits, supplying a missing strand for proper folding and assembly into functional pili.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Biochemistry

Background:

  • Gram-negative bacteria utilize adhesive pili for various functions, including host cell adhesion.
  • The biogenesis of these complex protein structures involves precise subunit assembly.
  • Periplasmic chaperones, such as PapD, play a critical role in this assembly process.

Purpose of the Study:

  • To elucidate the mechanism by which periplasmic PapD-like chaperones facilitate the assembly of adhesive pili.
  • To understand the role of chaperones in subunit folding and stabilization during pilus biogenesis.

Main Methods:

  • Investigated the interaction between PapD-like chaperones and pilus subunits.
  • Analyzed the structural contribution of chaperones to subunit folding.

Related Experiment Videos

  • Examined the process of subunit capping and stabilization by chaperones.

    • Main Results:

    • PapD-like chaperones bind and stabilize interactive surfaces of pilus subunits.
    • Chaperones supply a crucial seventh *gb-strand, enabling the completion of immunoglobulin-like folds in subunits.
    • Chaperones may act as templates, guiding subunit folding and providing steric information.

    Conclusions:

    • Periplasmic chaperones are essential for the correct folding and assembly of adhesive pili subunits.
    • Chaperones cap interactive surfaces, preventing premature assembly and ensuring proper organelle biogenesis.
    • This chaperone-mediated process offers insights into general principles of organelle assembly in bacteria.