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Pull-down of Calmodulin-binding Proteins
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Pull-down of Calmodulin-binding Proteins

Published on: January 23, 2012

Identification and characterization of calmodulin binding sites in cGMP-gated channel using surface plasmon resonance

K W Koch1

  • 1Institut für Biologische Informationsverarbeitung, Forschungszentrum Jülich, Germany.

Methods in Enzymology
|March 29, 2000
PubMed
Abstract

No abstract available in PubMed .

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Calmodulin-dependent Signaling01:16

Calmodulin-dependent Signaling

Calmodulin (CaM) is a calcium-binding protein in eukaryotes that controls various calcium-regulated cellular processes. It has four calcium-binding sites that bind calcium to form the calcium-calmodulin ( Ca2+-CaM) complex. GPCR stimulation increases the calcium levels in the cells that bind to CaM and induces a conformational change.
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...

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