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Single-Step Enrichment of a TAP-Tagged Histone Deacetylase of the Filamentous Fungus Aspergillus nidulans for Enzymatic Activity Assay
Published on: May 1, 2019
A simple and rapid method for the preparation of a cell-free extract with CCAAT-binding activity from filamentous
M Kato1, K Hayashi, T Kobayashi
1Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University, Japan. kato@agr.nagoya-u.ac.jp
Bioscience, Biotechnology, and Biochemistry
|March 29, 2000
Abstract:
A simple and rapid method for the preparation of a cell-free extract with the CCAAT-binding activity was established with Aspergillus nidulans as a model fungus. Proteins were extracted with 6 M guanidine hydrochloride directly from mycelia and renatured by dialysis. This method was found applicable to other filamentous fungi such as Aspergillus oryzae and Trichoderma viride.
