Preferential localization of varying forms of photoactive 1, 8-naphthalimide compounds within the atheromatous
K L Kloster1, M M Judy, J L Matthews
1University of South Dakota School of Medicine, Sioux Falls, South Dakota 57105, USA.
Background And Objective:
We are currently working with a novel class of photoactivated 4-amino substituted 1,8-naphthalimide compounds for tissue bonding. With promising results in other tissues, we are pursuing potential vascular applications. This study focused on determining the appropriate compound formulation(s), concentration, and exposure times to optimize penetration of the heterogeneous arterial wall.
Study Design/Materials And Methods:
Segments of atheromatous rabbit carotid artery were immersed in hydrophilic or lipophilic forms of the compound, then frozen, cryosectioned, and examined by confocal microscopy.
Results:
The hydrophilic compound exhibited preferential localization within the intima and media and limited presence in the adventitia. Conversely, the lipophilic compound concentrated in the intima and adventitia with virtual exclusion from the media. Exposure to both forms resulted in complete penetration of the arterial wall.
Conclusion:
These results extend our knowledge and permit a more practical approach to potential vascular applications using these photoactivated compounds for tissue bonding.
