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Fluorescence spectroscopy of normal, SV40-transformed human keratinocytes, and carcinoma cells
A J Papadopoulos1, N N Zhadin, M L Steinberg
1Department of Physics, The City College of the City University of New York, NY 10031, USA.
Cancer Biochemistry Biophysics
|March 30, 2000
Abstract:
Native fluorescence emission and excitation spectra of SV40 infected human keratinocytes, A431 and SCC324 carcinoma cells, and normal human keratinocytes were measured and compared. A difference in the intracellular metabolic state of NADH was found between the normal cells and the cancer or virus-transformed cells. The observed difference, namely an increased proportion of bound, mitochondrial NADH in the cancer and virus-infected cells, manifests as a blue spectral shift in the emission spectra.