Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cellulase hyperproducers constructed from polyploids of Lentinus edodes.

H Toyama1, N Toyama

  • 1Department of Food Science and Technology, Faculty of Horticulture, Minamikyushu University, Takanabe, Miyazaki, Japan.

Microbios
|March 30, 2000
PubMed
Summary

Researchers induced autopolyploidization in Lentinus edodes using colchicine, then employed benomyl to select for cellulase hyperproducers. A selected strain, L1, maintained high cellulase productivity across five generations, indicating genetic stability.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Proton Radiotherapy with Induction Chemotherapy for Locally Advanced Pancreatic Cancer: A Retrospective Analysis of Treatment Strategy and Outcomes.

Clinical oncology (Royal College of Radiologists (Great Britain))·2026
Same author

Prolonged anaphylactic circulatory failure caused by blood transfusion during general anaesthesia.

Anaesthesia reports·2025
Same author

Bicarbonate in Arteries Measured Preoperatively for Cadaveric Single-lung Transplantation is Related to Intraoperative Extra-Corporeal Membrane Oxygenation Use: A Retrospective Preliminary Study.

International journal of organ transplantation medicine·2022
Same author

Gastrointestinal: A case of a pancreatobiliary-type intraductal papillary mucinous neoplasm of the pancreas filling the main pancreatic duct without visible mucin secretion.

Journal of gastroenterology and hepatology·2021
Same author

Total tumour volume as a prognostic factor in patients with resectable colorectal cancer liver metastases.

BJS open·2020
Same author

Extracellular Vesicles of Stem Cells to Prevent BRONJ.

Journal of dental research·2020

Area of Science:

  • Mycology
  • Biotechnology
  • Enzyme Engineering

Background:

  • Lentinus edodes is a valuable edible mushroom with potential industrial applications.
  • Cellulase enzymes are crucial for biomass degradation and biofuel production.
  • Polyploidization can alter fungal metabolic capabilities.

Purpose of the Study:

  • To induce autopolyploidization in Lentinus edodes.
  • To develop a method for selecting cellulase hyperproducing strains.
  • To assess the stability of enhanced cellulase productivity.

Main Methods:

  • Treatment of Lentinus edodes mycelia with colchicine to induce autopolyploidization.
  • Subsequent treatment with benomyl, a haploidizing agent, to induce sector formation.
  • Selection of cellulase hyperproducing sectors from the resulting colonies.

Related Experiment Videos

  • Evaluation of cellulase productivity in selected strains over multiple generations.

    • Main Results:

    • Autopolyploidization was successfully induced in Lentinus edodes.
    • Benomyl treatment led to the formation of fan-shaped sectors.
    • Cellulase hyperproducers were effectively selected from these sectors.
    • The selected hyperproducer strain, L1, demonstrated stable cellulase productivity over five generations.

    Conclusions:

    • Colchicine and benomyl treatments provide a viable method for generating and selecting cellulase hyperproducers in Lentinus edodes.
    • The enhanced cellulase productivity in strain L1 is genetically stable.
    • This research offers a promising avenue for improving enzyme production in L. edodes for industrial applications.