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Updated: Oct 17, 2026

Expression and Purification of Nuclease-Free Oxygen Scavenger Protocatechuate 3,4-Dioxygenase
Published on: November 8, 2019
Myeloperoxidase-catalyzed formation of PCDD/F from chlorophenols
J Wittsiepe1, Y Kullmann, P Schrey
1Ruhr-Universität Bochum, Abteilung für Hygiene, Sozial- und Umweltmedizin, Germany. wittsiepe@hygiene.ruhr-uni-bochum.de
Abstract:
Chlorophenols (CP) are transformed in vitro to polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/F) by a biochemical-catalyzed oxidation. This is shown for 2,4,5-tri-, 2,3,4,6-tetra-and pentachlorophenol with myeloperoxidase recovered from human leucocytes in the presence of hydrogen peroxide. The yield, the reaction, and the PCDD/F-pattern found depend on the CP. The formation rates are in the micromol-per-mol range for all substrates. The experiments confirm the suspicion that a biochemical formation of PCDD/F from precursors such as CPs can take place in the human body and that this metabolic pathway may lead to a higher inner exposure with PCDD/F than is now assumed.
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