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Study of xanthine oxidase immobilized electrode based on modified graphite
E G Horozova1, N D Dimcheva, Z J Jordanova
1Department of Physical Chemistry, University of Plovdiv, Bulgaria. horozova@argon.uni-plovdiv.bg
Zeitschrift Fur Naturforschung. C, Journal of Biosciences
|March 30, 2000
Summary
An enzyme electrode was developed using immobilized xanthine oxidase on a graphite plate for xanthine detection. This biosensor offers a rapid and stable amperometric response to xanthine, showing potential for practical applications.
Area of Science:
- Biotechnology
- Electrochemistry
- Biosensors
Background:
- Xanthine oxidase is a key enzyme in purine metabolism.
- Enzyme immobilization is crucial for developing stable biosensors.
- Electrochemical modification of electrode surfaces enhances enzyme activity and stability.
Purpose of the Study:
- To develop a novel enzyme electrode for xanthine detection.
- To investigate the electrochemical properties of immobilized xanthine oxidase.
- To evaluate the performance and stability of the developed enzyme electrode.
Main Methods:
- Immobilization of xanthine oxidase onto an electrochemically modified graphite plate.
- Amperometric detection of xanthine based on H2O2 electrooxidation.
- Characterization of the enzyme electrode's linearity, response time, and storage stability.
Main Results:
- The enzyme electrode exhibited a linear amperometric response to xanthine up to 65 microM.
- A rapid response time of 2 minutes was achieved.
- The enzyme electrode retained 80% of its activity after three weeks of storage at room temperature.
Conclusions:
- The developed enzyme electrode is a sensitive and stable platform for xanthine detection.
- Electrochemical modification of graphite provides an effective method for enzyme immobilization.
- The biosensor demonstrates potential for practical applications in xanthine monitoring.