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Study of xanthine oxidase immobilized electrode based on modified graphite.

E G Horozova1, N D Dimcheva, Z J Jordanova

  • 1Department of Physical Chemistry, University of Plovdiv, Bulgaria. horozova@argon.uni-plovdiv.bg

Zeitschrift Fur Naturforschung. C, Journal of Biosciences
|March 30, 2000
PubMed
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An enzyme electrode was developed using immobilized xanthine oxidase on a graphite plate for xanthine detection. This biosensor offers a rapid and stable amperometric response to xanthine, showing potential for practical applications.

Area of Science:

  • Biotechnology
  • Electrochemistry
  • Biosensors

Background:

  • Xanthine oxidase is a key enzyme in purine metabolism.
  • Enzyme immobilization is crucial for developing stable biosensors.
  • Electrochemical modification of electrode surfaces enhances enzyme activity and stability.

Purpose of the Study:

  • To develop a novel enzyme electrode for xanthine detection.
  • To investigate the electrochemical properties of immobilized xanthine oxidase.
  • To evaluate the performance and stability of the developed enzyme electrode.

Main Methods:

  • Immobilization of xanthine oxidase onto an electrochemically modified graphite plate.
  • Amperometric detection of xanthine based on H2O2 electrooxidation.

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  • Characterization of the enzyme electrode's linearity, response time, and storage stability.

    • Main Results:

    • The enzyme electrode exhibited a linear amperometric response to xanthine up to 65 microM.
    • A rapid response time of 2 minutes was achieved.
    • The enzyme electrode retained 80% of its activity after three weeks of storage at room temperature.

    Conclusions:

    • The developed enzyme electrode is a sensitive and stable platform for xanthine detection.
    • Electrochemical modification of graphite provides an effective method for enzyme immobilization.
    • The biosensor demonstrates potential for practical applications in xanthine monitoring.