Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Submicrosecond real-time fluorescence sampling: application to protein folding.

J Ervin1, J Sabelko, M Gruebele

  • 1School of Chemical Sciences and Beckman Institute for Advanced Science and Technology, University of Illinois, Urbana 61801, USA.

Journal of Photochemistry and Photobiology. B, Biology
|March 30, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Author Correction: Anthropogenic modification of forests means only 40% of remaining forests have high ecosystem integrity.

Nature communications·2021
Same author

Anthropogenic modification of forests means only 40% of remaining forests have high ecosystem integrity.

Nature communications·2020
Same author

Crowding, Sticking, and Partial Folding of GTT WW Domain in a Small Cytoplasm Model.

The journal of physical chemistry. B·2020
Same author

Multi-scale dynamics at the glassy silica surface.

The Journal of chemical physics·2019
Same author

Competition of individual domain folding with inter-domain interaction in WW domain engineered repeat proteins.

Physical chemistry chemical physics : PCCP·2019
Same author

SEIS: Insight's Seismic Experiment for Internal Structure of Mars.

Space science reviews·2019
Same journal

Phytofabrication of Chitosan-MgO nanocomposites: Evaluation of their antioxidant, antibacterial, anticancer, and zebrafish embryo biocompatibility properties.

Journal of photochemistry and photobiology. B, Biology·2026
Same journal

Ocean acidification exacerbates UVR-induced inhibition of photosystem II and I in Corallina officinalis.

Journal of photochemistry and photobiology. B, Biology·2026
Same journal

Application of a sulfonic acid-modified NE probe with low-toxicity in a mouse model of pneumonia.

Journal of photochemistry and photobiology. B, Biology·2026
Same journal

Corrigendum to 'Wavelength-dependent photobiomodulation attenuates synovial inflammation in fibroblast-like synoviocytes and a collagenase-induced osteoarthritis model' [Journal of Photochemistry & Photobiology B: Biology Volume 272 (2025) 113276].

Journal of photochemistry and photobiology. B, Biology·2026
Same journal

On-demand H₂S delivery from NIR-activated dithiocarbamate-functionalized AuAg microdroplets for cellular anti-inflammation.

Journal of photochemistry and photobiology. B, Biology·2026
Same journal

Multi-omics approach identifies candidate phototaxis regulators in brown algae and reveals a novel kinase localized to the paraflagellar body.

Journal of photochemistry and photobiology. B, Biology·2026
See all related articles

Modern fluorescence techniques, enhanced by new hardware, enable cost-effective, sub-microsecond protein folding kinetic studies. Advanced analysis methods like singular value decomposition (SVD) and chi-analysis objectively detect complex folding behaviors.

Area of Science:

  • Biophysics
  • Biochemistry
  • Physical Chemistry

Background:

  • Time-resolved fluorescence detection is crucial for studying protein folding dynamics.
  • Traditional methods face limitations in kinetic time resolution and cost-effectiveness.

Purpose of the Study:

  • To review modern fluorescence techniques for protein folding studies.
  • To highlight recent technological advancements improving kinetic time resolution and cost-effectiveness.
  • To introduce advanced data analysis methods for interpreting folding kinetics.

Main Methods:

  • Review of modern fluorescence detection techniques.
  • Application of array photomultipliers, computer-controlled data gating, and long-memory multi-channel digitizers.
  • Analysis of fluorescence wavelength profiles and decay transients.

Related Experiment Videos

  • Utilizing singular value decomposition (SVD) and chi-analysis for kinetic data interpretation.

    • Main Results:

    • Achieved sub-microsecond kinetic time resolution for fluorescence detection.
    • Enabled cost-effective measurement of fluorescence profiles and decay transients over extended time scales.
    • Demonstrated the effectiveness of SVD and chi-analysis for dissecting complex protein folding kinetics.
    • Chi-analysis provides an objective approach for identifying nonexponential kinetics in two-state systems.

    Conclusions:

    • Recent technological improvements have significantly advanced time-resolved fluorescence spectroscopy for protein folding research.
    • These advancements allow for detailed, cost-effective kinetic analysis of protein folding.
    • Objective analysis methods like chi-analysis enhance the interpretation of complex folding pathways.