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Published on: April 17, 2012
Single-cell MALDI: a new tool for direct peptide profiling
L Li1, R W Garden, J V Sweedler
1Department of Chemistry and Beckman Institute, University of Illinois, Urbana, IL 61801, USA.
Abstract:
Matrix-assisted laser desorption-ionization (MALDI) mass spectrometry (MS) is a rapid and sensitive analytical approach that is well suited for obtaining molecular weights of peptides and proteins from complex samples. MALDI-MS can profile the peptides and proteins from single-cell and small tissue samples without the need for extensive sample preparation, except for the cell isolation and matrix application. Strategies for peptide identification and characterization of post-translational modifications are presented. Furthermore, several recent enhancements in MALDI-MS technology, including in situ peptide sequencing as well as the direct spatial mapping of peptides in cells and tissues are discussed.
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