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Integration of an immunosorbent assay system: analysis of secretory human immunoglobulin A on polystyrene beads in a

K Sato1, M Tokeshi, T Odake

  • 1Kanagawa Academy of Science and Technology, Japan.

Analytical Chemistry
|March 31, 2000
PubMed

An immunosorbent assay system was integrated into a glass microchip. Polystyrene beads were introduced into a microchannel, and then human secretory immunoglobulin A (s-IgA) adsorbed on the bead surface was reacted with colloidal gold conjugated anti-s-IgA antibody and detected by a thermal lens microscope. The scale merits of liquid microspace on the molecular behavior remarkably contributed to reduced assay time. The integration cut the time necessary for the antigen-antibody reaction by 1/90, thus shortening the overall analysis time from 24 h to less than 1 h. Moreover, troublesome operations required for conventional immunosorbent assays could be replaced by simple operations.

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