Related Experiment Video
Updated: Aug 12, 2026

Biomembrane Fabrication by the Solvent-assisted Lipid Bilayer (SALB) Method
Published on: December 1, 2015
Degree of solute inclusion in native beta-cyclodextrin: chromatographic approach
1Laboratoire de Chimie Analytique, Faculte de Pharmacie, Domaine de la Merci, La Tronche, France. eric.peyrin@ujf-grenoble.fr
Abstract:
The reversed-phase liquid chromatography retention and separation of a series of D,L dansyl amino acids were investigated over a wide range of salting-out agent (sucrose) concentrations using native beta-cyclodextrin as a chiral stationary phase. An original treatment was developed to determine the number of sucrose molecules (n) excluded from the solute-beta-cyclodextrin cavity interface when the analyte transfer occurred. Using the n values, the relative degrees of compound inclusion were calculated and correlated to the steric bulkiness of the solute. Thermodynamic parameter variations are discussed in relation to the inclusion degree of the dansyl amino acids. This numerical approach is a valuable tool to explore the steric effects implied in the host-guest complex formation.
More Related Videos
Related Concept Videos
Chromatography: Introduction
The phase in which the compounds linger or on which the compounds adsorb is called the stationary phase, whereas the mobile phase is the solvent that carries the solutes to be analyzed. In traditional column chromatography, the mixture flows through the stationary phase, and the compounds partition between the stationary and mobile phases...
Chromatographic Methods: Classification
Chromatographic techniques are typically named by...
Analyte Adsorption and Distribution
Optimizing Chromatographic Separations
Band broadening refers to spreading solute bands as they travel through the column. This broadening can impact resolution. Plate height (H) represents the length required for one theoretical plate. A lower plate height corresponds to...
Ion-Exchange Chromatography
Size-Exclusion Chromatography
Silica particles offer advantages such as rigidity,...

