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Published on: August 3, 2021
Preparation and use of a photoactivatable glucose-6-phosphate analogue
J Peng1, P Y Chen, R B Marchase
1Department of Medicinal Chemistry, The University of Utah, Salt Lake City 84112-5820, USA.
Abstract:
A benzophenone-containing derivative of glucose-6-phosphate, 6-[(3-([2,3-3H2]-p-benzoyl dihydrocinnamidoylpropyl-1-oxy)phosphoryl]-D-glucopyranose ([3H]BZDC-Glc-6-P) was synthesized and employed to photoaffinity label proteins on intact rat liver microsomes. The use of a non-photoactivatable, UV-transparent desoxy analogue of BZDC, named p-benzyldihydrocinnamoyl (BnDC), is introduced as a general method to achieve competition when hydrophilic ligands are modified with hydrophobic photophores.
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