Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Changes in rat muscle with compensatory overload occur in a sequential manner.

P C Macpherson¹, R E Thayer, C Rodgers

¹Faculty of Kinesiology, University of Western Ontario, London, Canada.

Acta Physiologica Hungarica

|March 31, 2000

Summary

Compensatory overload in rat skeletal muscle causes early changes in tropomyosin and myosin, indicating fiber type shifts and degeneration-regeneration. These protein alterations reflect both muscle repair and increased neural activity.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Abstracts of the 26th International Workshop on Clinical Pharmacology of HIV, Hepatitis and other Antiviral Drugs 2025, 3-4 September 2025, Amsterdam, the Netherlands.

British journal of clinical pharmacology·2025

Same author

Deletion of <i>Sod1</i> in Motor Neurons Exacerbates Age-Related Changes in Axons and Neuromuscular Junctions in Mice.

eNeuro·2023

Same author

Association between multimorbidity and undiagnosed obstructive sleep apnea severity and their impact on quality of life in men over 40 years old.

Global health, epidemiology and genomics·2018

Same author

Association between Body Mass Index and All-Cause Mortality among Oldest Old Chinese.

The journal of nutrition, health & aging·2018

Same author

Comparison of Health and Risk Factors of Older, Working-age Australians, Italians and Italian-born Migrants to Australia, with Data from an Italian (PASSI), and an Australian (SAMSS) Risk Factor Surveillance System.

Journal of immigrant and minority health·2017

Same author

Quality of life in patients with vitiligo using the Short Form-36.

The British journal of dermatology·2017

Area of Science:

Muscle physiology
Molecular biology
Skeletal muscle adaptation

Background:

Compensatory overload is a model to study skeletal muscle adaptation.
Myofibril protein composition is crucial for muscle function.

Purpose of the Study:

To investigate the time course of skeletal muscle myofibril protein changes during compensatory overload.
To correlate these protein changes with contractile properties.

Main Methods:

Compensatory overload induced in rat plantaris muscle via synergistic muscle ablation.
Analysis of myosin light chain (LC) and tropomyosin (TM) composition using electrophoresis.
Myofibrillar ATPase assays to assess contractile protein interactions.

Main Results:

Related Experiment Videos

Early decreases in alpha:beta TM ratio and myofibrillar ATPase activity within one week.
Shift from type II to type I fibers with increased slow myosin LC1 after 30 days.
TM subunit ratio returned to fast-twitch profile after 77 days.

Conclusions:

Early TM subunit changes and decreased force/ATPase activity suggest muscle fiber degeneration-regeneration.
Protein expression alterations reflect both regenerative processes and increased neuromuscular activity.
Skeletal muscle adaptation involves complex molecular and functional remodeling.