Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Vitamin B12 transporters.

G J Russell-Jones1, D H Alpers

  • 1C/- Biotech Australia Pty Ltd, Roseville, NSW, Australia.

Pharmaceutical Biotechnology
|April 1, 2000
PubMed
Summary
This summary is machine-generated.

Vitamin B12 (VB12) absorption involves a complex pathway with multiple binding molecules and receptors. Surprisingly, this intricate VB12 uptake mechanism can be utilized to improve oral delivery of other substances.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Assessing somatization in urologic chronic pelvic pain syndrome.

BMC urology·2019
Same author

Production of ectopic gastric intrinsic factor in gastric mucosa of humans with chronic gastritis.

Digestive diseases and sciences·2011
Same author

Reduction in inflammation in the footpad of carrageenan treated mice following the topical administration of anti-TNF molecules formulated in a micro-emulsion.

Journal of controlled release : official journal of the Controlled Release Society·2010
Same author

Postcrystallization Analysis of the Irreproducibility of the Human Intrinsic Factor-Cobalamin Complex Crystals.

Crystal growth & design·2009
Same author

Development of drugs for gastrointestinal motor disorders: translating science to clinical need.

Neurogastroenterology and motility·2008
Same author

Crystal structure of human intrinsic factor: cobalamin complex at 2.6-A resolution.

Proceedings of the National Academy of Sciences of the United States of America·2007
Same journal

Formulation development of protein dosage forms.

Pharmaceutical biotechnology·2002
Same journal

Regulatory considerations in the development of protein pharmaceuticals.

Pharmaceutical biotechnology·2002
Same journal

Quality assurance and quality control for biopharmaceutical products.

Pharmaceutical biotechnology·2002
Same journal

Fundamentals of freeze-drying.

Pharmaceutical biotechnology·2002
Same journal

Membrane filtration.

Pharmaceutical biotechnology·2002
Same journal

Fundamentals of thermal sterilization processes.

Pharmaceutical biotechnology·2002
See all related articles

Area of Science:

  • Biochemistry
  • Cell Biology
  • Gastroenterology

Background:

  • Vitamin B12 (VB12) absorption is a highly complex physiological process.
  • It involves sequential binding of VB12 to haptocorrin and intrinsic factor (IF), followed by receptor-mediated uptake.
  • This intricate mechanism ensures efficient VB12 delivery into the bloodstream.

Purpose of the Study:

  • To elucidate the detailed molecular interactions in vitamin B12 intestinal uptake.
  • To explore the potential of leveraging the VB12 absorption pathway for enhanced oral delivery of other molecules.

Main Methods:

  • Detailed analysis of the sequential binding of VB12 to haptocorrin and intrinsic factor (IF).
  • Identification of specific receptors involved in intestinal epithelial cell uptake of the IF-VB12 complex.

Related Experiment Videos

  • Investigation of the role of transcobalamin II (TcII) in VB12 transport across cells.

    • Main Results:

    • The VB12 uptake pathway involves at least five distinct VB12-binding molecules, receptors, and transporters.
    • Each component exhibits unique affinity and specificity for VB12 and interacts with separate cell receptors.
    • The process includes proteolytic release of VB12 and subsequent binding to TcII for circulation.

    Conclusions:

    • Despite its complexity, the vitamin B12 uptake mechanism presents a unique opportunity.
    • This pathway can be harnessed to enhance the oral bioavailability of peptides, proteins, and nanoparticles.
    • Further research can optimize this strategy for therapeutic and nutritional applications.