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[Sequence alterations in p53 gene of hepatocellular carcinoma from high aflatoxin risk area in Guangxi]
Objective:
Check on the sequence alterations of the p53 gene of hepatocellular carcinoma (HCC) in some areas of Guangxi.
Methods:
40 cases of archival HCC samples were divided into two groups. One includes 26 cases from Fusui--an area notorious for high risk in HCC, AFB1 and HBV; The other includes 14 cases from Yulin where both HCC and HBV were high but AFB1 may be low because rice low in AFB1 was the staple food. Exon 7 were sequenced by DNA direct sequencing. p53 mutational protein and HBsAg were examined by immunostaing.
Results:
In the Fusui group, 16/26 (61.5%) cases showed mutation at codon 249 third position G to T transversion to form a hot-spot which is generally considered to be caused by AFB1. In the Yulin group, only one single place-Guigang showed p53 mutation clustered at the hot-spot like Fusui in 3/5 cases (60%), while the other cases demonstrated mutational points in various positions.
Conclusion:
Besides Qidong and South Africa, a third AFB1 high risk area was demonstrated where HCC is accompanied by p53 gene specific mutation.
