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[Effect of eosinophil major basic protein on histamine release from nasal epithelial mast cells]
Abstract:
Eosinophils and nasal epithelial mast cells (NEMCs) are closely related in nasal allergy. The activity of the eosinophil cationic protein, major basic protein (MBP) on histamine release from human NEMCs has not been documented. We examined the effects of MBP on histamine release from NEMCs. Nasal scrapings (containing NEMCs) from 59 patients with house dust mite nasal allergy were incubated with MBP or alkylated MBP. Specimens were also pre-incubated with MBP followed by incubation with house dust mite allergen (HDM) or preincubated with HDM followed by incubation with MBP or HDM. Histamine released in incubated media and remained in incubated mast cells was quantified by radio-immunoassay. MBP at the concentration of 10(-5) mol/L induced mild but statistically significant histamine release from the NEMCs. Preincubation with MBP at the concentration of 1.57 x 10(-5) mol/L produced slight inhibition of subsequent HDM-induced histamine release but preincubation with HDM showed no effect on the subsequent MBP-induced or HDM-induced histamine release from NEMCs. These results suggest that accumulated and activated eosinophils may release MBP resulting in clinical symptoms by interaction with NEMCs and inhibit of histamine release from NEMCs by subsequent interaction with allergen.
Insights
Major basic protein (MBP) from eosinophils causes mild histamine release from nasal mast cells. MBP may also inhibit allergen-induced histamine release, potentially impacting nasal allergy symptoms.
Area of Science:
- Immunology
- Allergy Research
- Cell Biology
Context:
- Nasal allergy involves complex interactions between eosinophils and nasal epithelial mast cells (NEMCs).
- The specific role of eosinophil major basic protein (MBP) in modulating NEMC activity, particularly histamine release, remains largely uncharacterized.
Purpose:
- To investigate the direct effects of eosinophil major basic protein (MBP) on histamine release from human NEMCs.
- To determine if MBP influences histamine release induced by house dust mite allergen (HDM).
Summary:
- Human NEMCs isolated from allergic patients were incubated with MBP or alkylated MBP.
- MBP (10^-5 mol/L) induced a statistically significant, albeit mild, histamine release from NEMCs.
- Pre-incubation with MBP partially inhibited subsequent HDM-induced histamine release, while HDM pre-incubation did not affect MBP or HDM-induced histamine release.
Impact:
- These findings suggest that eosinophil-derived MBP can directly interact with NEMCs, contributing to allergic inflammation.
- MBP's potential inhibitory effect on allergen-induced histamine release warrants further investigation for therapeutic implications in nasal allergies.