[Isolation, purification and activity measurement of proteolytic enzymes in dental plaque]

The purpose of this study was to investigate the types and activities of proteolytic enzymes in human root surface plaque. Sephadex G-200 gel filtration, fluorometry and spectrometry were used to isolate, purify and demonstrate the activities of proteolytic enzymes. The results indicated that three enzymes were present in the root surface plaque, namely leucine amino peptidase, dipeptidyl peptidase i.v. and trypsin-like proteinase, and their activities were different.