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A modified staining method for connective tissue in semi-thin histological sections of peripheral and cranial nerves
T Menovsky1, M van den Bergh Weerman
1Department of Neurosurgery, University Hospital Nijmegen St. Radboud, The Netherlands.
Acta Chirurgiae Plasticae
|April 1, 2000
Abstract:
A simple staining method for collagen in semi-thin sections of nerves is described. This technique consists of applying basic fuchsin (0.05%) solution for 2 min. to +/- 1.25 microns plastic resin-embedded sections after routine toluidine blue staining. This staining method clearly demonstrates the amount and orientation of collagenous connective tissue in the nerve, both in transverse and longitudinal sections.