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Size-exclusion chromatography study of the molecular-weight distribution of gamma-irradiated pullulan

K I Shingel1, V M Tsarenkov, P T Petrov

  • 1Scientific Pharmaceutical Centre, Belmedpreparaty Pharmaceutical Co., Minsk, Belarus.

Carbohydrate Research
|April 1, 2000
PubMed

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Size-Exclusion Chromatography01:08

Size-Exclusion Chromatography

In size-exclusion chromatography (SEC), also known as molecular-exclusion or gel-permeation chromatography, molecules are separated based on their sizes. This technique is important for separating large molecules such as polymers and biomolecules. The two classes of micron-sized stationary phases encountered in SEC are silica particles and cross-linked polymer resin beads. Both materials are porous, but their pore sizes vary significantly.
Silica particles offer advantages such as rigidity,...