Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Reverse relationship between mitogen activated protein kinase and human platelet aggregation.

S V Kansra1, S D Shukla

  • 1Department of Pharmacology, School of Medicine, University of Missouri-Columbia, 65212, USA.

Clinical and Experimental Hypertension (New York, N.Y. : 1993)
|April 1, 2000
PubMed
Summary

Low temperatures increase mitogen-activated protein kinase (MAPK) activity in human platelets, but decrease their aggregation response. This suggests a reverse relationship between MAPK and platelet aggregation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Neuroprotective effects of Withania somnifera Dunn. in hippocampal sub-regions of female albino rat.

Phytotherapy research : PTR·2001
Same author

Ethanol and lipid metabolic signaling.

Alcoholism, clinical and experimental research·2001
Same author

Stress induced neuron degeneration and protective effects of Semecarpus anacardium Linn. and Withania somnifera Dunn. in hippocampus of albino rats: an ultrastructural study.

Indian journal of experimental biology·2001
Same author

Metabolic fate of [14C]-ethanol into endothelial cell phospholipids including platelet-activating factor, sphingomyelin and phosphatidylethanol.

Journal of biomedical science·2001
Same author

Selective secretion of protein kinase C isozymes by thrombin-stimulated human platelets.

Biochemical and biophysical research communications·2001
Same author

Nuclear activation and translocation of mitogen-activated protein kinases modulated by ethanol in embryonic liver cells.

Biochimica et biophysica acta·2000

Area of Science:

  • Biochemistry
  • Hematology
  • Cellular Biology

Background:

  • Platelets play a crucial role in hemostasis and thrombosis.
  • Mitogen-activated protein kinases (MAPKs) are involved in various cellular signaling pathways.
  • The specific role of MAPK in platelet function, particularly in relation to temperature, is not fully understood.

Purpose of the Study:

  • To investigate the role of MAPK in human platelets.
  • To determine the effect of temperature on MAPK activity and platelet aggregation.
  • To explore the relationship between MAPK activity and platelet aggregation.

Main Methods:

  • Human platelets were maintained at different temperatures (4°C, 15°C, and 37°C).
  • MAPK activity was measured using biochemical assays.

Related Experiment Videos

  • Platelet aggregation was induced by thrombin and monitored.
  • Experiments were conducted on intact platelets and platelet cytosol.

    • Main Results:

    • MAPK activity in intact platelets increased approximately twofold at 4°C compared to 37°C.
    • Platelet aggregation induced by thrombin decreased significantly at 4°C (85%) and 15°C (71%).
    • MAPK activity in platelet cytosol decreased at 4°C, indicating temperature effects are specific to intact platelets.
    • Reversibility of MAPK activity was observed upon returning platelets from 4°C to 37°C.

    Conclusions:

    • Temperature significantly influences MAPK activity and aggregation in human platelets.
    • A potential inverse correlation exists between MAPK activity and platelet aggregation.
    • These findings suggest MAPK plays a regulatory role in temperature-dependent platelet responses.