Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Microsomal triglyceride transfer protein expression during mouse development.

J M Shelton1, M H Lee, J A Richardson

  • 1Division of Endocrinology, Diabetes, and Medical Genetics, Medical University of South Carolina, 171 Ashley Avenue, Charleston, SC 29425-2222, USA.

Journal of Lipid Research
|April 1, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Using dust shed from asteroids as microsamples to link remote measurements with meteorite classes.

Meteoritics & planetary science·2020
Same author

Municipal Wastewater as a Microbial Surveillance Platform for Enteric Diseases: A Case Study for Salmonella and Salmonellosis.

Environmental science & technology·2018
Same author

Radiation promotes colorectal cancer initiation and progression by inducing senescence-associated inflammatory responses.

Oncogene·2015
Same author

Description of a new non-injectable connector to reduce the complications of arterial blood sampling.

Anaesthesia·2014
Same author

The effects of necrotic enteritis, aflatoxin B1, and virginiamycin on growth performance, necrotic enteritis lesion scores, and mortality in young broilers.

Poultry science·2013
Same author

Guidance of dorsal root ganglion neurites and Schwann cells by isolated Schwann cell topography on poly(dimethyl siloxane) conduits and films.

Journal of neural engineering·2011
Same journal

The Metabolic-Immune Axis in MASH: Is the Macrophage Signature the Key to Liver Disease Staging?

Journal of lipid research·2026
Same journal

BK channel deficiency promotes lipolysis via an AKT-independent activation of the cAMP/PKA/HSL pathway.

Journal of lipid research·2026
Same journal

Interleukin-6 stimulates platelet 12-lipoxygenase to drive coagulation in inflammatory arthritis.

Journal of lipid research·2026
Same journal

LIPA regulates myeloid differentiation and is essential for intra-plaque macrophage accumulation during atherogenesis.

Journal of lipid research·2026
Same journal

Hydrolysis and depletion of phosphatidylglycerol at peak murine acute lung injury.

Journal of lipid research·2026
Same journal

Multispecies transcriptome analysis identifies networks protecting against MASLD in Göttingen minipigs.

Journal of lipid research·2026
See all related articles

Microsomal triglyceride transfer protein (MTP) is crucial for embryonic development, with expression detected early in yolk sac and liver tissues. This pattern suggests MTP

Area of Science:

  • Developmental Biology
  • Lipid Metabolism
  • Molecular Genetics

Background:

  • Feto-maternal transfer of lipophilic nutrients is vital for fetal development, potentially mediated by lipoproteins.
  • Apolipoprotein B (apoB) and microsomal triglyceride transfer protein (MTP) are key proteins in lipoprotein secretion.
  • Genetic deficiencies in humans are not linked to embryonic lethality, but MTP knockout mice exhibit early embryonic lethality.

Purpose of the Study:

  • To investigate the ontogeny and spatial expression pattern of MTP during mouse embryonic development.
  • To correlate MTP expression patterns with its proposed role in nutrient transfer.

Main Methods:

  • In situ hybridization was employed to analyze MTP gene expression.
  • Microwave heating of tissue sections was used to optimize hybridization efficiency.

Related Experiment Videos

  • Expression patterns were examined across various embryonic time points and tissues.

    • Main Results:

    • Robust MTP expression was observed in yolk sac tissues starting early in development.
    • Primordial liver cell nests showed MTP expression by embryonic day 9.5 (E9.5).
    • Intestinal MTP expression began around E12.5, reaching adult levels by E14.5; no expression was found in heart, kidney, placenta, or maternal decidua.

    Conclusions:

    • The temporal and spatial expression pattern of MTP supports its role in facilitating lipophilic nutrient transfer.
    • MTP likely mediates nutrient transfer from the yolk sac to the developing embryo before liver maturation.
    • Post-establishment of the circulatory system, MTP facilitates transfer to the liver.