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Related Experiment Videos

Albumin purification from human placenta.

J Cabrera-Crespo1, V M Gonçalves, E A Martins

  • 1Centro de Biotecnologia, Instituto Butantan, Av. Vital Brasil 1500, CEP 05503-900, São Paulo, SP, Brazil.

Biotechnology and Applied Biochemistry
|April 4, 2000
PubMed
Summary

This study details an industrial method for purifying albumin from human placenta, yielding high-purity therapeutic albumin. The process ensures product stability and safety, offering an alternative to plasma-derived albumin.

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Area of Science:

  • Biochemistry
  • Biotechnology
  • Pharmaceutical Manufacturing

Background:

  • Human albumin is a crucial therapeutic protein.
  • Placental blood offers an alternative source for albumin purification compared to plasma.
  • Developing efficient industrial-scale purification processes is essential.

Purpose of the Study:

  • To describe an industrial process for purifying albumin from human placenta.
  • To evaluate the yield, purity, and stability of the purified placental albumin.
  • To establish a safe and effective alternative albumin source.

Main Methods:

  • Industrial-scale albumin purification from 50 kg of human placentas.
  • Key steps include saline extraction, selective precipitation, ultrafiltration, thermo-coagulation, activated charcoal treatment, chromatography (anion-exchange and hydrophobic-interaction), and pasteurization.

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  • Utilized basket centrifugation, press filtration, and 30 kDa cross-flow ultrafiltration (CFUF) membranes.

    • Main Results:

    • Achieved an average yield of 4.5 g of albumin per kg of placenta.
    • The purified albumin demonstrated high purity (97.1%) with low A(403) (0.05).
    • The final product passed in vivo pyrogen and toxicity tests and showed no polymers or aggregates post-stability testing.

    Conclusions:

    • The described industrial process effectively purifies albumin from human placenta.
    • The resulting albumin meets stringent purity and safety standards, comparable to plasma-derived products.
    • Placental albumin represents a viable and safe alternative for therapeutic applications.