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Advances in microbial amylases.

A Pandey1, P Nigam, C R Soccol

  • 1Laboratorio de Processos Biotecnologicos, Departamento de Engenharia Quimica, Universidade Federal do Parana (UFPR), CEP 81531-970 Curitiba-PR, Brazil.

Biotechnology and Applied Biochemistry
|April 4, 2000
PubMed
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This review surveys microbial amylases (alpha-amylase, beta-amylase, glucoamylase), highlighting their industrial significance and diverse applications in biotechnology, pharmaceuticals, and beyond. Microbial sources meet industrial demands, offering potential for new applications.

Area of Science:

  • Biotechnology
  • Enzymology
  • Microbiology

Background:

  • Amylases are crucial enzymes with significant roles in modern biotechnology.
  • While sourced from plants, animals, and microorganisms, microbial amylases are preferred for industrial applications.
  • Their utility extends to pharmaceuticals, fine chemicals, clinical, medicinal, and analytical chemistry.

Purpose of the Study:

  • To provide a comprehensive survey of microbial amylases, including alpha-amylase, beta-amylase, and glucoamylase.
  • To discuss the molecular biology, production, and properties of these enzymes.
  • To explore the expanding applications of amylases in various industries.

Main Methods:

  • Literature review of microbial amylase sources, molecular biology, production, and properties.

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  • Discussion of enzyme structures, cloning, sequences, and genetic modification techniques like protoplast fusion and mutagenesis.

    • Main Results:

    • Microbial sources are generally preferred for industrial amylase production.
    • Amylases have broad applications in starch saccharification, food, brewing, distilling, textile, pharmaceutical, and chemical industries.
    • Advances in biotechnology are continually expanding the application spectrum of amylases.

    Conclusions:

    • Microbial amylases are vital industrial enzymes with diverse and growing applications.
    • Further research into enzyme properties and production can unlock new potential in pharmaceuticals and fine chemicals.
    • Understanding amylase molecular biology is key to optimizing their use in biotechnology.