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Related Concept Videos

Bacterial RNA Polymerase00:43

Bacterial RNA Polymerase

Unlike eukaryotes, bacteria use a single RNA Polymerase (RNAP) to transcribe all genes. The different subunits of bacterial RNAPhave distinct functions. The multisubunit structure of the bacterial RNAP helps the enzyme to maintain catalytic function, facilitate assembly, interact with DNA and RNA, and self-regulate its activity.
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
Transcription Attenuation in Prokaryotes02:42

Transcription Attenuation in Prokaryotes

Transcriptional attenuation occurs when RNA transcription is prematurely terminated due to the formation of a terminator mRNA hairpin structure.  Bacteria use these hairpins to regulate the transcription process and control the synthesis of several amino acids including histidine, lysine, threonine, and phenylalanine. Transcription attenuation takes place in the non-coding regions of mRNA.
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
Bacterial Transcription01:53

Bacterial Transcription

RNA polymerase (RNAP) carries out DNA-dependent RNA synthesis in both bacteria and eukaryotes. Bacteria do not have a membrane-bound nucleus. So, transcription and translation occur simultaneously, on the same DNA template.
Transcription can be divided into three main stages, each involving distinct DNA sequences to guide the polymerase. These are:
Bacterial RNA Polymerase00:43

Bacterial RNA Polymerase

Unlike eukaryotes, bacteria use a single RNA Polymerase (RNAP) to transcribe all genes. The different subunits of bacterial RNAPhave distinct functions. The multisubunit structure of the bacterial RNAP helps the enzyme to maintain catalytic function, facilitate assembly, interact with DNA and RNA, and self-regulate its activity.
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
Transcription in Prokaryotes01:28

Transcription in Prokaryotes

Transcription is a highly regulated process that converts genetic information into RNA molecules. The transcription cycle is divided into three key stages: initiation, elongation, and termination, each driven by specific molecular mechanisms.Initiation of TranscriptionIn bacteria, transcription begins when the RNA polymerase core enzyme associates with a sigma factor to form a holoenzyme. For example, the E. coli sigma factor called σ70 forms a holoenzyme, which recognizes the -10 (Pribnow box)...
Coordination of Gene Expression Processes in Bacteria01:29

Coordination of Gene Expression Processes in Bacteria

The DNA replication, transcription, and translation processes are intricately coupled in bacteria, allowing efficient gene expression and rapid protein synthesis. While this physical and functional coordination is advantageous, it introduces challenges that bacteria overcome through specific regulatory mechanisms.Coupling of Replication, Transcription, and TranslationThe coupling of replication, transcription, and translation is a hallmark of bacterial gene expression. As the replisome unwinds...

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Sensing metabolic signals with nascent RNA transcripts: the T box and S box riboswitches as paradigms.

Cold Spring Harbor symposia on quantitative biology·2007
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The GA motif: an RNA element common to bacterial antitermination systems, rRNA, and eukaryotic RNAs.

RNA (New York, N.Y.)·2001
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Transcriptional activation of the Bacillus subtilis ackA promoter requires sequences upstream of the CcpA binding site.

Journal of bacteriology·2001
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Bacillus subtilis ccpA gene mutants specifically defective in activation of acetoin biosynthesis.

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tRNA determinants for transcription antitermination of the Bacillus subtilis tyrS gene.

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Related Experiment Video

Updated: Jul 12, 2026

Rapid Verification of Terminators Using the pGR-Blue Plasmid and Golden Gate Assembly
09:51

Rapid Verification of Terminators Using the pGR-Blue Plasmid and Golden Gate Assembly

Published on: April 25, 2016

Transcription termination control in bacteria.

T M Henkin1

  • 1Department of Microbiology, The Ohio State University, Columbus, OH 43210, USA. henkin.3@osu.edu

Current Opinion in Microbiology
|April 4, 2000
PubMed
Summary

Transcription termination is a controlled, dynamic process. Recent molecular insights into elongation, termination, and protein-RNA interactions enhance our understanding of its regulation.

Area of Science:

  • Molecular Biology
  • Gene Regulation

Background:

  • Transcription termination is a critical regulatory step in gene expression.
  • This process is known to be complex and influenced by multiple regulatory mechanisms.

Purpose of the Study:

  • To elucidate the molecular mechanisms governing transcription elongation and termination.
  • To deepen the understanding of protein-RNA interactions in controlling transcription termination.

Main Methods:

  • Review and synthesis of recent literature on transcription termination.
  • Analysis of molecular mechanisms involved in transcription elongation and termination control.

Main Results:

  • Emerging data reveal dynamic control points in transcription termination.

More Related Videos

Analysis of Termination of Transcription Using BrUTP-strand-specific Transcription Run-on (TRO) Approach
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Analysis of Termination of Transcription Using BrUTP-strand-specific Transcription Run-on (TRO) Approach

Published on: March 12, 2017

Application of Biolayer Interferometry (BLI) for Studying Protein-Protein Interactions in Transcription
07:18

Application of Biolayer Interferometry (BLI) for Studying Protein-Protein Interactions in Transcription

Published on: July 26, 2019

Related Experiment Videos

Last Updated: Jul 12, 2026

Rapid Verification of Terminators Using the pGR-Blue Plasmid and Golden Gate Assembly
09:51

Rapid Verification of Terminators Using the pGR-Blue Plasmid and Golden Gate Assembly

Published on: April 25, 2016

Analysis of Termination of Transcription Using BrUTP-strand-specific Transcription Run-on (TRO) Approach
12:12

Analysis of Termination of Transcription Using BrUTP-strand-specific Transcription Run-on (TRO) Approach

Published on: March 12, 2017

Application of Biolayer Interferometry (BLI) for Studying Protein-Protein Interactions in Transcription
07:18

Application of Biolayer Interferometry (BLI) for Studying Protein-Protein Interactions in Transcription

Published on: July 26, 2019

  • New findings highlight the significance of protein-RNA interactions in this process.

    • Conclusions:

    • A comprehensive framework for understanding transcription termination control is developing.
    • Further research into molecular details will refine our knowledge of gene regulation.