Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Characterization of Trypanozoon isolates using a repeated coding sequence and microsatellite markers.

Biteau1, Bringaud, Gibson

  • 1Laboratoire de Parasitologie Moléculaire, Université Victor Segalen de Bordeaux II, UPRESA-5016 CNRS, 146 Rue Léo Saignat, 33076, Bordeaux, France

Molecular and Biochemical Parasitology
|April 4, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Demonstrated Two Sections of the Auriculo-Ventricular Bundle.

Transactions. Medico-Chirurgical Society of Edinburgh·2018
Same author

Pain in the leg [endocarditis subacute bacterial].

Case reports. Children's Memorial Hospital (Chicago, Ill.)·2010
Same author

Irritability; excessive perspiration.

Case reports. Children's Memorial Hospital (Chicago, Ill.)·2010
Same author

Intermittent cough [bronchiectasis; lobectomy].

Case reports. Children's Memorial Hospital (Chicago, Ill.)·2010
Same author

Fever; convulsions [pneumococcic meningitis in 6 weeks old infant; recovery].

Case reports. Children's Memorial Hospital (Chicago, Ill.)·2010
Same author

Generalized swelling.

Case reports. Children's Memorial Hospital (Chicago, Ill.)·2010

Genetic markers reveal significant diversity within Trypanosoma species, enabling differentiation. A specific genetic pattern was identified that distinguishes the human pathogen Trypanosoma brucei gambiense group 1.

Area of Science:

  • Genetics
  • Molecular Biology
  • Parasitology

Background:

  • Microsatellite loci are crucial for genetic studies like linkage analysis and population comparisons.
  • The Trypanosoma (Trypanozoon) brucei complex includes significant animal and human pathogens.

Purpose of the Study:

  • To analyze genetic variation in Trypanosoma species using microsatellites and a repeated DNA sequence.
  • To identify genetic markers for differentiating Trypanosoma species and subspecies, particularly human-pathogenic forms.

Main Methods:

  • Analysis of a repeated DNA coding sequence and eleven novel microsatellites.
  • Genotyping of ninety-seven isolates from five Trypanosoma species/subspecies: T. evansi, T. equiperdum, T. b. brucei, T. b. rhodesiense, and T. b. gambiense.

Related Experiment Videos

Main Results:

  • High genetic heterogeneity observed in the repeated coding sequence and five microsatellites.
  • Several markers exhibited high polymorphism, allowing for group-specific genotypes.
  • A distinct genetic pattern was identified that specifically segregates Trypanosoma brucei gambiense group 1.

Conclusions:

  • Microsatellite markers are effective tools for characterizing genetic diversity in Trypanosoma.
  • The identified markers can aid in the classification and identification of Trypanosoma species and subspecies.
  • A specific genetic marker can reliably distinguish the human pathogen Trypanosoma brucei gambiense group 1.