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Updated: Aug 9, 2026

High-throughput Gene Tagging in Trypanosoma brucei
Published on: August 12, 2016
Characterization of Trypanozoon isolates using a repeated coding sequence and microsatellite markers
1Laboratoire de Parasitologie Moléculaire, Université Victor Segalen de Bordeaux II, UPRESA-5016 CNRS, 146 Rue Léo Saignat, 33076, Bordeaux, France
Abstract:
Genetic variation of microsatellite loci is a widely used method for linkage analysis, individual identification or inter-population studies. Here we analyse a repeated DNA coding sequence and eleven new microsatellites identified within the Trypanosoma (Trypanozoon) brucei genome. Ninety-seven isolates belonging to the five species and subspecies Trypanosoma evansi, T. equiperdum, T. brucei brucei, T. b. rhodesiense and T. b. gambiense were compared regarding the genetic patterns of these markers. The results reveal a great heterogeneity of the genotypes related to the repeated coding sequence and five microsatellites, some of which show a high degree of polymorphism. This allows us to define group-specific genotypes or alleles; in particular, we show that one specific pattern clearly segregates the human pathogen T. b. gambiense group 1.
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