The effects of long-term heparin application on ACh-induced isolated ileum contractility and structure

The present study was designed to investigate the side-effects of long-term (one and two months), overdoses (1000 and 2000 IU/kg/day) heparin (Liquamine) applications on the isolated ileum contractility and the histopathological alterations in the ileal tissues. The histopathology of tissues was investigated by using light microscopy. Isolated ileum contractility was measured by using the conventional organ bath system with standard and Ca-free Tyrode perfusion solutions. Ileum preparations were initially contracted with ACh maximally and these contraction amplitudes were given as% values. The average amplitude of these contractions of all experimental groups were found to be increased significantly with respect to control group, in both perfusion solutions. Under light microscopy, in the preparations treated with 1000 IU/kg/day heparin, we have observed edema in the ileal mucosa and neutrophil infiltration in the villi. In addition, the glandular tissue degeneration was also seen in 2000 IU/kg@day group. We can suggest that most probably, the binding of heparin to the receptor on the cell membrane results Ca-release.